shigeta

11,687 reputation

939

bio	website	affymetrix.com/analysis
	location	Berkeley, CA
	age	49
visits	member for	1 year, 5 months
	seen	5 hours ago
stats	profile views	133

PhD structural biology. I do bioinformatics for Affymetrix in the SF bay area. Interested in systems / synthetic biology, expression analysis and machine learning.

	11,687 reputation	bio	website	affymetrix.com/analysis	visits	member for	1 year, 5 months
939 badges		location	Berkeley, CA		seen	5 hours ago

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6h	comment	Has medical progress stopped human evolution? here's a post if not the primary reference. This sounds reasonable to me, though i'm sure its not completely proven. abc.net.au/news/2009-07-29/…
6h	revised	Specifically What Protein Folding Calculations does Folding@Home do? clarified title
6h	comment	Specifically What Protein Folding Calculations does Folding@Home do? I see someone did it - i will vote to reopen.
9h	comment	How difficult is to renature a protein? those antibodies would not be fully denatured. just enough chaotropic agent probably to disrupt protein-protein interactions to get the antibodies off the column. Antibodies have one of the more stable folds and will not usually denature under such conditions. if you did I would be surprised if you could reconstitute a functionally folded protein... imho
9h	comment	How to use mechanical microstrainer to extract tissue proteins from human? thanks - never used it so can't comment - sounds cleaner and more $$$ than a hydraulic press, a bead beater or other similar methods.. :)
16h	comment	How difficult is to renature a protein? my impression has been that only relatively small proteins (~10kDa) are readily renatured and used in protein folding kinetics experiments. Its pretty unlikely that anything larger will be useful. Do you have any specific examples of a larger protein that has been refolded by any means, and if so do you think this is a typical result?
16h	comment	Specifically What Protein Folding Calculations does Folding@Home do? suggest: "The Folding@Home project claims to solve protein folding problem on participant's desktops when they're not in use. Could anyone tell me what the calculations are that are running through Folding@Home and in general how protein folding calculations work? An example of a problem solved through Folding@Home would be especially appreciated."
2d	comment	How to use mechanical microstrainer to extract tissue proteins from human? what is a micro strainer? is this an idea you have or a reference to a specific piece of equipment? several methods of the sort you have described may be used, but i've not heard of a microstrainer...
2d	comment	Is local equilibrium a reasonable assumption for evolutionary processes? You are right - overall the environment, which is always subject to change puts individuals in dynamic equilibrium. See the Red Queen hypothesis. The genome itself is never completely at equilibrium either.
2d	revised	What determines a journal's 'impact factor'? edited tags
May 16	comment	How is transport of glucose into prokaryotic cells different from transport into eukaryotic cells? Interestingly, bacterial and eukaryotic glucose transporters are similar in structure, though bacteria do not have insulin. ncbi.nlm.nih.gov/pubmed/23075985
May 15	answered	If Evolution Is In Progress, Why Fight Extinction?
May 15	answered	Recent and good quality articles on systems biology
May 15	awarded	Nice Answer
May 14	comment	Why do ants dig surface paths? I wonder if this might be an extension of nest-building behavior. the dirt that runs along both sides of the path looks like debris from nest cleaning.
May 14	comment	Why is a slow worm not considered a snake? the defining factor is that the species definition comes from where they species has come from, not only its body plan. In fact the slow worm has features in its body plan that more resemble lizards than snakes. The species name is as much a reflection of the history of that animal as it is a description of what may look like.
May 14	comment	How is the exogenous DNA protected from degradation during bacterial transformation? As an aside, one of the reasons that plasmids are circular no doubt is to prevent natural degradation of the sort you are talking about.
May 14	revised	What could account for an apparent loss of mass at death? added 14 characters in body
May 13	revised	Does the human ear adapt to noise levels? title seemed confusing - hope this is what you meant
May 13	answered	What's the difference between shotgun sequencing and clone based sequencing?