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bio website benmil.es
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visits member for 1 year, 11 months
seen Feb 26 '13 at 17:00

Jun
7
answered Why don't teeth glow?
Jun
4
comment Troubleshooting bioconjugates migration in a SDS-PAGE gel?
I see, thank you for explaining.
Jun
4
awarded  Commentator
Jun
4
comment Troubleshooting bioconjugates migration in a SDS-PAGE gel?
where would the poly-disperse radius of gyration arise from if the molecules are coated with SDS?
May
30
comment What makes a wet dog so stinky?
This is kind of a physical chemistry question really as it is related to the evaporation of volatile compounds rather than biochemistry specifically.
May
30
answered What makes a wet dog so stinky?
May
26
comment Herbal vs Chemical Insecticides
All insecticides are chemicals by the definition of a chemical.
May
7
answered What are key factors when evaluating and comparing miniprep columns?
May
1
asked How many agarose gel bands are typical for circularised DNA
May
1
comment Does anyone have any TOPO directional cloning tips?
great advice thank you.
May
1
accepted Does anyone have any TOPO directional cloning tips?
Apr
30
comment Does anyone have any TOPO directional cloning tips?
thanks @bobthejoe. I suppose you are correct it is more of a point of discussion, apologies for that. The original cloning underwent trouble shooting and the problem could could still not be solved. This question can be closed.
Apr
30
comment Does anyone have any TOPO directional cloning tips?
Ah sorry I should clarify, I first tried the traditional approach to cloning by inserting restriction sites either side of my gene via PCR then double digesting the PCR product and target vector. Following double digestion I then attempted a ligation but I nor my colleagues could get any positive transformants. So I am moving to a different method of cloning using the TOPO kit and I am wondering if anyone has any pointers before I begin using it. It is the pET101/D-TOPO kit.
Apr
30
asked Does anyone have any TOPO directional cloning tips?
Apr
27
awarded  Supporter
Apr
27
answered Genetic effects on personality
Apr
26
awarded  Scholar
Apr
26
accepted Primer design for introduction of restriction sites flanking a gene of interest
Apr
26
comment Primer design for introduction of restriction sites flanking a gene of interest
Great answers thank you guys
Apr
26
comment Primer design for introduction of restriction sites flanking a gene of interest
Great answers thank you guys