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Jun 4 |
awarded | Commentator |
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Jun 4 |
comment |
Troubleshooting bioconjugates migration in a SDS-PAGE gel? where would the poly-disperse radius of gyration arise from if the molecules are coated with SDS? |
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May 30 |
comment |
What makes a wet dog so stinky? This is kind of a physical chemistry question really as it is related to the evaporation of volatile compounds rather than biochemistry specifically. |
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May 30 |
answered | What makes a wet dog so stinky? |
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May 26 |
comment |
Herbal vs Chemical Insecticides All insecticides are chemicals by the definition of a chemical. |
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May 7 |
answered | What are key factors when evaluating and comparing miniprep columns? |
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May 1 |
asked | How many agarose gel bands are typical for circularised DNA |
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May 1 |
comment |
Does anyone have any TOPO directional cloning tips? great advice thank you. |
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May 1 |
accepted | Does anyone have any TOPO directional cloning tips? |
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Apr 30 |
comment |
Does anyone have any TOPO directional cloning tips? thanks @bobthejoe. I suppose you are correct it is more of a point of discussion, apologies for that. The original cloning underwent trouble shooting and the problem could could still not be solved. This question can be closed. |
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Apr 30 |
comment |
Does anyone have any TOPO directional cloning tips? Ah sorry I should clarify, I first tried the traditional approach to cloning by inserting restriction sites either side of my gene via PCR then double digesting the PCR product and target vector. Following double digestion I then attempted a ligation but I nor my colleagues could get any positive transformants. So I am moving to a different method of cloning using the TOPO kit and I am wondering if anyone has any pointers before I begin using it. It is the pET101/D-TOPO kit. |
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Apr 30 |
asked | Does anyone have any TOPO directional cloning tips? |
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Apr 27 |
awarded | Supporter |
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Apr 27 |
answered | Genetic effects on personality |
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Apr 26 |
awarded | Scholar |
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Apr 26 |
accepted | Primer design for introduction of restriction sites flanking a gene of interest |
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Apr 26 |
comment |
Primer design for introduction of restriction sites flanking a gene of interest Great answers thank you guys |
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Apr 26 |
comment |
Primer design for introduction of restriction sites flanking a gene of interest Great answers thank you guys |
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Apr 25 |
comment |
Majority of transcripts are from sense strand? You are indeed correct, as the antisense strand is used as the template to make the mRNA translation of the DNA sense strand. |
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Apr 25 |
awarded | Student |
