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Jul 11 |
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Is there a detectable amount of bacterial DNA in the blood of infected persons? Yeah I think qPCR is a fairly routine diagnostic for determining a strain. |
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Jul 10 |
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Why do we sweat after drinking water and running? @gpuguy Do you mean you sweat after exercise even if you drink water? Or do you mean that at rest, in a cool room when one wouldn't usually sweat, you drink a lot of water and then begin to sweat? |
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Jun 28 |
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How do I deal with sticky and viscous samples from cell lysates? Just to say I have the same problem! So I look forward to the answer. |
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Jun 28 |
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Why is the Kakapo more attracted to humans than its own kind? @Richard_Smith Great answer. |
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Jun 8 |
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Why are we using upper teeth and lower lip on labiodental sounds? It definitely feels like an unusual jaw movement to do it with your lower teeth. The jaw is required to laterally move forward rather than longitudinally move, which is a more common movement in terms of other vocalisations and eating. |
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Jun 4 |
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Troubleshooting bioconjugates migration in a SDS-PAGE gel? I see, thank you for explaining. |
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Jun 4 |
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Troubleshooting bioconjugates migration in a SDS-PAGE gel? where would the poly-disperse radius of gyration arise from if the molecules are coated with SDS? |
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May 30 |
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What makes a wet dog so stinky? This is kind of a physical chemistry question really as it is related to the evaporation of volatile compounds rather than biochemistry specifically. |
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May 26 |
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Herbal vs Chemical Insecticides All insecticides are chemicals by the definition of a chemical. |
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May 1 |
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Does anyone have any TOPO directional cloning tips? great advice thank you. |
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Apr 30 |
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Does anyone have any TOPO directional cloning tips? thanks @bobthejoe. I suppose you are correct it is more of a point of discussion, apologies for that. The original cloning underwent trouble shooting and the problem could could still not be solved. This question can be closed. |
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Apr 30 |
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Does anyone have any TOPO directional cloning tips? Ah sorry I should clarify, I first tried the traditional approach to cloning by inserting restriction sites either side of my gene via PCR then double digesting the PCR product and target vector. Following double digestion I then attempted a ligation but I nor my colleagues could get any positive transformants. So I am moving to a different method of cloning using the TOPO kit and I am wondering if anyone has any pointers before I begin using it. It is the pET101/D-TOPO kit. |
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Apr 26 |
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Primer design for introduction of restriction sites flanking a gene of interest Great answers thank you guys |
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Apr 26 |
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Primer design for introduction of restriction sites flanking a gene of interest Great answers thank you guys |
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Apr 25 |
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Majority of transcripts are from sense strand? You are indeed correct, as the antisense strand is used as the template to make the mRNA translation of the DNA sense strand. |
