4,027 reputation
1431
bio website youtube.com/user/Arm0ry
location London, UK / Stuttgart, Germany
age 23
visits member for 1 years, 11 months
seen 7 hours ago

Studying BSc Biomedical Science at Imperial College London.

I have several years worth of experience in

web development (HTML, PHP, CSS, JS, SQL; both raw and using frameworks jQuery and Smarty)

and programming (Windows/C++, Android/Java, Flash/AS3)

as well as some hobbyist experience in design (Inkscape, GIMP, Autodesk 3D).

Active member of the London Hackspace Biohackers whenever time permits; I also love tweaking and meddling but rarely find the time these days (in other words, hacking). Also, laptops don't permit for meddling a lot unfortunately.


Nov
20
comment What can cause incompatible sticky ends to be ligated?
Sounds like a valid explanation and is probably not even too unlikely. Unfortunately I haven't got access to the plasmid sequences anymore so I can't verify it :(
Oct
20
awarded  Nice Question
Oct
1
revised Is this a valid principle of curing HIV?
added 153 characters in body
Oct
1
revised Is this a valid principle of curing HIV?
edited title
Oct
1
revised Is this a valid principle of curing HIV?
added 40 characters in body
Oct
1
comment Need of X or Y chromosome protein after meiosis
I can't think of a particular reason why there would be a need for genes involved in spermatogenesis to be located on the X or Y, all genes involved could simply be on autosomes. I don't know whether any are though (or aren't).
Sep
30
comment Need of X or Y chromosome protein after meiosis
Are you asking what proteins are exclusive to X and Y from all other chromosomes or are you asking which proteins are on the X but not the Y (and vice versa)? These are two very different questions.
Sep
30
revised Is this a valid principle of curing HIV?
added 459 characters in body
Sep
30
comment Is this a valid principle of curing HIV?
Here's a list of FDA-approved drugs against HIV: fda.gov/ForConsumers/byAudience/ForPatientAdvocates/…
Sep
30
comment Is this a valid principle of curing HIV?
The question shows lack of some basic information on current HIV treatment, which is completely fine in my opinion. Afterall we're here to answer questions. In the end, the question is simply asking what's the problem with treating HIV at the moment.
Sep
30
answered Is this a valid principle of curing HIV?
Sep
19
comment How many consecutive cell divisions are required to form the adult human body from the single cell zygote?
The Hayflick limit is entirely unrelated to this issue as the foetus develops firstly from embryonic totipotent stem cells, then pluripotent stem cells, and even in the adult body there are still multipotent stem cells, some of which are capable of indefinite replication.
Sep
16
comment What happens if an IV drip with a saline solution slips out of the vein, but keeps dripping into the body?
Is this a question about a real case? Or are you possibly asking about a homework question? (It sounds kind of like that to me)
Sep
7
comment How to calculate virus titre from qPCR
The initial prep is 293Ts in a medium with the viral plasmid as well as packaging and envelope plasmids; cells are perforated (but not killed) by adding PEI which leads to production of functional virus. That's then harvested, aliquoted out into stock vials and frozen. I was told it's not possible to just directly qPCR the aliquots - I assume the viral proteins are the problem. I used one of the vials to infect cells with decreasing concentrations of it, extracted their DNA after three days and qPCR'd that in order to analyse how many viruses actually integrated into the cellular genome.
Sep
6
reviewed Approve suggested edit on pharmacokinetic tag wiki excerpt
Sep
6
reviewed Approve suggested edit on pharmacodynamic tag wiki excerpt
Sep
5
comment How to calculate virus titre from qPCR
I think it makes more sense to qPCR the lysates for copies, with all virions removed, as that should give me an idea of how many cells a mL of virus stock can transfect, which I believe is actually the important factor - I don't think I really need to know exactly how many viral particles that mL contains in order to transfect that many cells. Does that make sense?
Sep
5
comment How to calculate virus titre from qPCR
Thanks for the reply. I see that I left out relevant information so I added that - have a read of the first paragraph again and let me know if that makes more sense now :)
Sep
5
revised How to calculate virus titre from qPCR
added 327 characters in body
Sep
4
comment Why we have no enzyme to digest cellulose?
Evolution doesn't make everything perfect.