4,027 reputation
1431
bio website youtube.com/user/Arm0ry
location London, UK / Stuttgart, Germany
age 23
visits member for 1 year, 11 months
seen yesterday

Studying BSc Biomedical Science at Imperial College London.

I have several years worth of experience in

web development (HTML, PHP, CSS, JS, SQL; both raw and using frameworks jQuery and Smarty)

and programming (Windows/C++, Android/Java, Flash/AS3)

as well as some hobbyist experience in design (Inkscape, GIMP, Autodesk 3D).

Active member of the London Hackspace Biohackers whenever time permits; I also love tweaking and meddling but rarely find the time these days (in other words, hacking). Also, laptops don't permit for meddling a lot unfortunately.


Mar
20
comment In which order did the cells of the immune system evolve?
Information being interesting or useful for medical students doesn't necessarily make it applicable to medicine ;)
Mar
11
comment Organs lifespan out of the body
It sounds like what you are really asking is "What organ can be conserved outside of the body for the longest time and still function when reimplanted?" - Is that correct? If so, please edit your question to reflect that and provide more detail!
Feb
25
comment What is immunosuppression? Why would one use it?
Referring to immunosuppression in cancer, I would expect him to be thinking of leukaemias or lymphomas. Immunosuppression is actually quite common there firstly because the cancerous cells are most often (in lymphomas always) themselves immune cells and directly affected by immunosuppressive treatment - on the other hand it is a typical part of bone marrow transplantation procedures, which are sometimes the best or only treatment for these conditions.
Feb
16
comment What does 5' and 3' mean in DNA and RNA strands?
The five carbons in the ribose backbone are numbered starting from the O in clockwise direction* - therefore the carbon which has the base (A, T, C, G) attached is called 1'. The 2' carbon does nothing special, the 3' carbon has an OH group (downwards dash in the diagram), the 4' carbon connects the ribose ring to 1' via the O bond, and the 5' carbon is outside of the ring, attached to the 4' carbon on one side and phosphate on the other. PS: *) strictly, it's not after the ring direction but rather it comes from the linear configuration, i.e. when ribose is not in ring form.
Feb
4
comment How do duplicate brain regions (ex: left/right amygdaloid body) operate together?
The two symmetrical counterparts of a brain compartment can interact with each other through connections in the corpus callosum. The purpose behind two regions of the same kind differs. For example, often it is to handle inputs from the two sides of the body respectively. Check out en.wikipedia.org/wiki/Lateralization_of_brain_function to get started.
Nov
20
comment What can cause incompatible sticky ends to be ligated?
Sounds like a valid explanation and is probably not even too unlikely. Unfortunately I haven't got access to the plasmid sequences anymore so I can't verify it :(
Oct
1
comment Need of X or Y chromosome protein after meiosis
I can't think of a particular reason why there would be a need for genes involved in spermatogenesis to be located on the X or Y, all genes involved could simply be on autosomes. I don't know whether any are though (or aren't).
Sep
30
comment Need of X or Y chromosome protein after meiosis
Are you asking what proteins are exclusive to X and Y from all other chromosomes or are you asking which proteins are on the X but not the Y (and vice versa)? These are two very different questions.
Sep
30
comment Is this a valid principle of curing HIV?
Here's a list of FDA-approved drugs against HIV: fda.gov/ForConsumers/byAudience/ForPatientAdvocates/…
Sep
30
comment Is this a valid principle of curing HIV?
The question shows lack of some basic information on current HIV treatment, which is completely fine in my opinion. Afterall we're here to answer questions. In the end, the question is simply asking what's the problem with treating HIV at the moment.
Sep
19
comment How many consecutive cell divisions are required to form the adult human body from the single cell zygote?
The Hayflick limit is entirely unrelated to this issue as the foetus develops firstly from embryonic totipotent stem cells, then pluripotent stem cells, and even in the adult body there are still multipotent stem cells, some of which are capable of indefinite replication.
Sep
16
comment What happens if an IV drip with a saline solution slips out of the vein, but keeps dripping into the body?
Is this a question about a real case? Or are you possibly asking about a homework question? (It sounds kind of like that to me)
Sep
7
comment How to calculate virus titre from qPCR
The initial prep is 293Ts in a medium with the viral plasmid as well as packaging and envelope plasmids; cells are perforated (but not killed) by adding PEI which leads to production of functional virus. That's then harvested, aliquoted out into stock vials and frozen. I was told it's not possible to just directly qPCR the aliquots - I assume the viral proteins are the problem. I used one of the vials to infect cells with decreasing concentrations of it, extracted their DNA after three days and qPCR'd that in order to analyse how many viruses actually integrated into the cellular genome.
Sep
5
comment How to calculate virus titre from qPCR
I think it makes more sense to qPCR the lysates for copies, with all virions removed, as that should give me an idea of how many cells a mL of virus stock can transfect, which I believe is actually the important factor - I don't think I really need to know exactly how many viral particles that mL contains in order to transfect that many cells. Does that make sense?
Sep
5
comment How to calculate virus titre from qPCR
Thanks for the reply. I see that I left out relevant information so I added that - have a read of the first paragraph again and let me know if that makes more sense now :)
Sep
4
comment Why we have no enzyme to digest cellulose?
Evolution doesn't make everything perfect.
Sep
4
comment Strongest muscle of Human Body
First internet search result gives you everything you may be interested in, and looks reasonably reliable: loc.gov/rr/scitech/mysteries/muscles.html
Aug
22
comment What can cause incompatible sticky ends to be ligated?
We actually digested EcoRI and XmaI twice using different buffers (B and MC) to see which worked better, but the results were pretty identical (and two bands easily distinguishable) so it seems that was fine. I analysed 12 of the 22 cultures on my plate, and of those 12, 4 (33%) carried the short re-ligated variant B. That seems a lot to me for a rare event.
Aug
21
comment What can cause incompatible sticky ends to be ligated?
Thanks for the hint. I added those and rewrote the description a bit, hopefully that's clearer now.
Aug
19
comment What can cause incompatible sticky ends to be ligated?
Incomplete digestion at what point do you mean? If the backbone (p1) was incompletely digested before the ligation, it should have produced a larger band than the one which I excised from the gel. If the analytical digest after ligation was incomplete, I would expect to see at least faint bands in addition to the one clear band corresponding to the backbone size.