168 reputation
6
bio website
location Netherlands
age
visits member for 2 years, 7 months
seen Nov 4 at 12:53

Nov
3
comment Providing small molecules to cells on a filter plate
The original poster would like to feed his immobilized cells small molecules, without fixing or waiting for adherence. The inserts contain a membrane, which forms a permeable barrier, to allow diffusion of small molecules, and site for attachment. The insert itself is free to move and hang in different culture media/wells, so no need to trypsinize or resolubilize them, and provides containment of the non-attached cells.
Oct
25
accepted How does translational coupling work in prokaryotes?
Oct
25
answered Providing small molecules to cells on a filter plate
Apr
10
awarded  Nice Question
Feb
18
asked Why prefer $MgSO_4$ over $MgCl_2$ in recipies
Nov
19
awarded  Popular Question
Nov
29
answered Low temperature PCR
Oct
9
answered Sparking during electroporation of plasmid DNA into bacterial cells
Jul
5
answered Why did high A+T content create problems for the Plasmodium falciparum genome project?
Jul
5
awarded  Teacher
Jul
4
answered Deletion errors with Phusion Polymerase?
Jun
4
awarded  Supporter
Jun
4
awarded  Scholar
Jun
4
accepted Why is proteinase K digestion performed at 50 °C?
May
17
asked Why is proteinase K digestion performed at 50 °C?
May
11
awarded  Student
May
11
asked How does translational coupling work in prokaryotes?