168 reputation
6
bio website
location Netherlands
age
visits member for 2 years, 7 months
seen Nov 4 at 12:53

Nov
3
comment Providing small molecules to cells on a filter plate
The original poster would like to feed his immobilized cells small molecules, without fixing or waiting for adherence. The inserts contain a membrane, which forms a permeable barrier, to allow diffusion of small molecules, and site for attachment. The insert itself is free to move and hang in different culture media/wells, so no need to trypsinize or resolubilize them, and provides containment of the non-attached cells.