MattDMo

1,293 reputation

124

bio	website	pigimal.com
	location	Massachusetts
	age	34
visits	member for	11 months
	seen	15 mins ago
stats	profile views	9

Long-time OSS user/admin, beginning serious coder - I heart Python. Master of Science in Molecular Medicine, many years' experience in biotech working with antibodies and related assays. Science and computer geek. Dad.

	1,293 reputation	bio	website	pigimal.com	visits	member for	11 months
124 badges		location	Massachusetts		seen	15 mins ago

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3h	comment	What mammalian cell lines are easiest to grow and transfect? good luck finding truly diploid cell lines - unless you're working with primary cells, most cell lines are transformed, and have all sorts of genetic anomalies.
14h	reviewed	Reopen Specifically What Protein Folding Calculations does Folding@Home do?
1d	reviewed	Approve suggested edit on Writing methods section on PCR amplication in a paper
1d	comment	How difficult is to renature a protein? @shigeta - I was specifically thinking of antibodies (150 kDa, 4 subunits disulfide-bonded to each other). At my previous job we experimented with purifying antibodies by eluting them from an antigen column with varying concentrations of guanidine, then dialyzing directly into PBS. We didn't do any biophysical characterization to look at the extent of denaturation, but in our functional assays they performed similarly to those eluted by the standard (pH-dependent) method.
May 17	revised	How difficult is to renature a protein? edited tags
May 17	answered	How difficult is to renature a protein?
May 17	comment	Writing methods section on PCR amplication in a paper Tag should probably be Taq...
May 17	reviewed	Close What molecules/elements/compounds are in the cardiovascular system?
May 17	comment	Writing methods section on PCR amplication in a paper You should change your "Amplification..." sentence to remove the word "condition" and change 25 ml to 25 µL, but other than that it looks okay. Also, maybe spell out "Forward" and "Reverse" when listing your primers.
May 17	reviewed	Reviewed Primer design for Gibson assembly
May 17	revised	Primer design for Gibson assembly formatting, link to Gibson assembly
May 16	comment	What material is recommended for autoradiography cassettes when working with 32P? If you're worried, contact your local radiation safety committee - they should have a wide-spectrum detector around that can detect Bremsstrahlung. I've only ever used aluminum/plastic cassettes myself for autorads. The phosphor screen should block a good deal, but you do not want to take any chances where radiation is concerned...
May 14	revised	Modularity of transcription factors added 446 characters in body
May 14	reviewed	Close Recent and good quality articles on systems biology
May 14	reviewed	Close Basic book on Human brain anatomy
May 13	answered	Breeding laboratory mice with cancer; how does this work?
May 13	revised	SNPs mapping into protein deleted 2 characters in body; edited title
May 13	comment	SNPs mapping into protein thank you for using one of my favorite gene names :)
May 13	comment	SNPs mapping into protein I assume SNP means Single-Nucleotide Polymorphism? I'm confused exactly what your question is - how does one take a SNP and see what effect it has on the protein sequence? Is that it?
May 12	revised	Is it possible to print humans? edited body; edited tags