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1h
comment Why can't proteins make copies of themselves?
Because they have no "blueprint" to work off of. DNA is the "set of directions" used to make new proteins.
1h
comment What makes DNA sequences most different/recognizable from a biological perspective?
That's going to vary from one nucleic acid binding protein to another. Remember, DNA and RNA are not simply sequences of letters, but there can be secondary structure, as well as more basic chemical interactions between adjacent or more distant bases - see RNA hairpins as an example. All of this gives a "shape" to the recognized portion of nucleic acid, which can inform binding capacity just as much as, or potentially even more than primary sequence alone.
7h
comment Could potatoes that have been fridged for 142 days still sprout afterwards?
This question is probably better suited to Gardening & Landscaping.
1d
comment Wasp ID(in Ohio)?
I'm voting to close this question as off-topic because without a clear image, exact identification is impossible.
1d
comment Do transcripts always start and end with exons?
@David just as a point of clarification, users are under no obligation whatsoever to explain or "justify" their votes, up or down. The message you are referring to appears to low-rep or casual users of a site, but not to more experienced users, who theoretically understand the voting guidelines. I would also point out, as March Ho did, that lashing out in such a defensive and insulting manner, is neither useful to the overall tone of the site, nor likely to prompt anyone to explain or change their vote. Be nice.
2d
comment Does drinking alcohol having UTI may help to kill the bacteria?
Good answer, but I would get rid of the last paragraph. The concentration of ethanol in urine is never going to be high enough to kill even a small percentage of bacteria present. In the United States, a person is legally drunk if their blood alcohol content (BAC) is between 0.08% and 0.1%, depending on the state. Even assuming that unmetabolized alcohol is concentrated 10-fold, and a person is very drunk with a BAC of 0.3%, 3% EtOH won't kill much of anything, especially if it's in a biofilm, as UTIs are.
2d
comment B-cell antibody production
I'm not sure how "different" got in there, I suspect it may be a translation issue or something similar. I suppose the BCRs are "different" in that they're different individual molecules, perhaps what the OP meant to say. I don't know... :)
2d
comment Why does pETDuet-1 only have one T7 terminator but two T7 promoters?
Could they be thinking that the transcription complex from ORF1 would fall apart when hitting an new transcription complex that is being assembled on promoter/RBS 2?
2d
comment Why does pETDuet-1 only have one T7 terminator but two T7 promoters?
Sounds like the manufacturer could answer these questions much better than we can.
2d
comment B-cell antibody production
Please see my comment above. Statement 2 is true, you just misunderstood the question.
2d
revised B-cell antibody production
added 15 characters in body
2d
comment B-cell antibody production
The specificity of antibodies is answered below. However, I think the answerer misunderstood the "250000" number. What this actually refers to is the number of copies of the cell's antibody that are on the cell surface - each antibody-producing B cell has approximately 250000 antibody molecules on its surface. Each one of those copies is identical, all recognizing exactly the same epitope.
Apr
25
comment Cell Lines in Cancer Biology
Some of this info is on ATCC's or DSMZ's websites, others you will have to get from papers. What exactly do you mean by "mechanism of action" and "advantages and disadvantages over one another"? Drugs have a mechanism of action, cells don't. The advantages and disadvantages of using one particular cell line over another are completely experiment-dependent, so there's no possible way anyone could foresee what you're doing without your telling us.
Apr
24
comment Alkali Biuret Test
General chemistry questions are off-topic here, but can be asked on Chemistry.
Apr
24
comment Why do calluses appear most frequent on ring finger?
Do you have any evidence to show that this is a common occurrence, or is this just your personal experience? Everyone develops calluses differently, if they develop them at all, depending on what they use their hands for on a regular basis.
Apr
24
comment How big advantage had a trait have to bring to evolve in the human evolution?
"we still grow many unnecessary organs" - huh? Which organs are unnecessary? The liver? Kidneys? Stomach?
Apr
24
comment Why I cannot find dendritic cells in blood smear?
This was by regular flow cytometry, so I wasn't taking images of them. All the images I've seen of DC in vivo are along the lines of these - the staining is somewhat diffuse because of all the ruffles and extensions. If you do a search for "mature dendritic cell SEM" you'll find a bunch of scanning electron microscopy images of the cells in 3D.
Apr
24
revised Voltage sensitive dyes technique: What is the underlying measure?
deleted 1 character in body
Apr
24
answered Voltage sensitive dyes technique: What is the underlying measure?
Apr
24
comment Why do many people seem to prefer unhealthy compared to healthy foods?
You had me at "deep fried cheese covered bacon"