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5h
comment What is the concentration of leptin in white adipose tissue in mice?
Have you attempted any research on your own before asking strangers on the internet?
18h
comment What concentration of table salt is needed to kill bacteria in sweaty gym clothes?
You should keep in mind that the odor is a mixture of stale sweat and chemicals produced by microorganisms, not necessarily the microorganisms themselves. Basically, by the time the odors appear, it's already too late. Thorough washing and immediate drying of your clothes after exercising can help mediate the accumulation of additional odors, but what's there is there. These aromatic organic compounds can bind very tightly to the fibers of the cloth, and are quite difficult to get rid of. Bleach may help, depending on the type of fabric (and color), but I doubt salt will do much of anything.
18h
revised Why does my anti-ubiquitin antibody visualization not work on my PAGE gel?
deleted 2 characters in body; edited title; edited tags
18h
comment Why does my anti-ubiquitin antibody visualization not work on my PAGE gel?
Do you have protease inhibitors in your lysis buffer? If so, which ones? Also, does your anti-ubiquitin antibody work when doing a straight SDS-PAGE Western blot?
19h
awarded  Popular Question
2d
comment What did the transition from ovipary to Hemotrophic viviparity look like?
Most evolutionary changes happened slowly over countless generations. This statement is by no means universally agreed-upon. Read about punctuated equilibrium for one of the major competing theories.
2d
comment How to be a Noah-esque biopirate and store the genes of every plant and animal
@GoodGravy you have to keep in mind that with humans, all of the hard work has already been done. There already exists reference genomes, databases of SNPs, programs for (essentially) automated assembly, etc. You can quickly sequence and get meaningful data out of a human sample quite quickly. However, imagine a completely unknown mushroom, for example. You can probably get 20X coverage (or whatever you need nowadays) of reads. But how do you then assemble, and more importantly annotate it? We have templates for humans, but not necessarily for any random species out there.
Jun
26
comment Why do human have continuously-growing hair on their head?
@SolarLunix check out en.wikipedia.org/wiki/Timeline_of_human_evolution and en.wikipedia.org/wiki/Human_evolution for starters. And, BTW, Homo is a genus, not a phylum.
Jun
26
comment Why do human have continuously-growing hair on their head?
@SolarLunix there is no such thing as the "missing link". It's a fallacy dating back to the ancient Greeks, and used by creationists to attempt to disprove evolution. Look it up.
Jun
26
comment is left brachiochephalic vein and left pulmonary artery is same?
Veins are not the same as arteries. A non-capillary blood vessel is either one or the other, not both.
Jun
26
comment Why does human hair grow over our eyes
I'm sure late hominids/early humans were intelligent enough to brush their hair back from their faces. Also, what exactly do you mean by "natural humans"? Modern Homo sapiens is just as "natural" as Homo neanderthalensis, Homo erectus, and our other ancestors. Stone Age humans are just as "natural" as 21st century humans.
Jun
26
revised Southern blotting
added 15 characters in body
Jun
26
comment Southern blotting
@Transcriptase you may want to consider posting a link to either an open-access journal or some other website. Not everyone has access to Nature.
Jun
26
comment Immortality Gene? Really?
I looked up the subject of the interview you linked at livescience.com on PubMed, and looked back to about 2010 before giving up. There are 121 citations in that search, and nearly all of them are in Rejuvenation Research, a journal that he just happens to be the editor of. All of the references I looked up were quite obviously editorials, so it looks like this guy is just playing at being a scientist. Maybe he's good at synthesizing current research, but he sure isn't contributing anything original.
Jun
23
awarded  Constituent
Jun
20
comment best book for a comprehensive introduction to biology for a computer science graduate interested in undertaking research in bioinformatics
I concur with biogirl. MBotC was my introduction to molecular biology, and will give you a lot of background information. Of course, it shouldn't be your only resource (you wouldn't read just one book to learn a new programming language, for example), but it's a great place to start.
Jun
19
comment Cell Line With DNMT3a Mutation
All I'm saying is if you can't find a cell line that meets your needs, it would be quite cost-effective to generate one on your own. There are some extra costs outside of the kit, but this Life Technologies kit is only US$514, whereas purchasing a new cell line from ATCC not in your collection may cost an approximately equivalent amount, depending on the line, what kind of institution you're at, etc. Worth thinking about.
Jun
19
comment Cell Line With DNMT3a Mutation
Why not just induce one? There are quite easy-to-use CRISPR/Cas kits available nowadays.
Jun
19
awarded  Caucus
Jun
19
comment Has anyone tried to prepare chemically competent Mycobacteria?
Mycobacteria are very different from E. coli. I would be rather surprised if you could adapt the methods without a lot of customization. What's wrong with electroporation?