4,338 reputation
1233
bio website mattdmo.com
location near Boston
age 36
visits member for 2 years, 10 months
seen 1 hour ago

Long-time OSS user/admin, intermediate coder - I heart . Master of Science in Molecular Medicine, many years' experience in biotech working with antibodies and cell-based assays. Science and computer geek. Dad.


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Mar
27
comment R Tool and analysis of Gene Expression data from NCBI GEO datasets
I'm voting to close this question as off-topic because coding questions belong on Stack Overflow.
Mar
25
comment How do BCAA's promote faster muscle recovery?
What are BCAAs? Please define acronyms when posting questions (and answers) here.
Mar
24
comment Primer Designing
Have you tried googling? There are many resources out there.
Mar
22
comment How are Stem Cells Produced for Stem Cell Based Therapies?
Can you give a reference to a particular therapy you're interested in? Different treatments use different protocols.
Mar
21
comment Should gene therapy safety protocol include isolation?
@Nederealm no, there's only one vector, delivered with a completely replication-deficient virus. Isolation of gene therapy patients is completely unnecessary.
Mar
21
comment Should gene therapy safety protocol include isolation?
@user137 you don't use lentiviral vectors in AIDS patients.
Mar
19
comment What are the lower and upper hearing limits of the human ear?
Decibel limit for what? The lowest discernible sound? That depends on frequency, age of listener, etc. The level at which damage occurs? What kind of damage? The level at which deafness occurs? How quickly are you talking about? Seconds? Minutes? Instantaneously? Basically, please edit your question (don't respond in the comments) and clarify what exactly you're looking for. Otherwise, this question may be closed as "Too Broad" or "Unclear What You're Asking".
Mar
16
comment By what mechanism does Risperidone swell breast tissue?
Could you add a reference for this?
Mar
14
comment Do transcription factors bind to both strands of DNA?
I don't think this is a duplicate, in fact I might vote to close the other question and link to this one, as the accepted answer is much clearer and nicely illustrated, with links to sources. However, both questions are fundamentally different, and neither necessarily needs to be closed. Yes, they are about similar things, but are not identical.
Mar
14
comment What type of flask should I use to culture NTERA2 embryonic cancer stem cells?
I just use regular TC-treated Petri dishes and flasks - nothing special.
Mar
10
comment Monochromator in fluorescence microscopy
Well, according to Cold Spring Harbor Protocols, xenon arc lamp/monochromator-based fluorescence microscopes are economical, performant, and rather common.
Mar
8
comment Name two reasons why it is impossible to determine a gene's nucleotide sequence from the amino acid sequence of the polypeptide
@MarchHo well, I didn't exactly give an answer, though, just pointed the OP in the right direction. Vaguely.
Mar
8
comment Name two reasons why it is impossible to determine a gene's nucleotide sequence from the amino acid sequence of the polypeptide
Think carefully about how a gene's sequence is turned into protein - the gene is transcribed, mRNA is created, it runs through the ribosome where tRNAs match the codons and bring in the appropriate amino acids, then the polypeptide chain is spit out. Is there anywhere in that series of steps where the sequence of bases written in DNA could somehow change? Are there any spots where bases might be "clipped off" for some reason?
Mar
3
comment Why do haemophillic females $X^hX^h$ die before birth?
I also think this question should remain open. It doesn't match any of the close reasons, is narrowly scoped and perfectly answerable. We address incorrect information all the time, that's one of the reasons this site is here.
Feb
23
comment Why have whales and dolphins not evolved to have gills?
@Cliff and the answer is: just because that's the way things happened.
Feb
23
comment Why is blood removed from meat?
Right, it's your guess, which is why I asked for references.
Feb
23
comment Why is blood removed from meat?
Can you add some references, especially for your last sentence?
Feb
20
comment Can a portion of human skin cells be modified in some way to generate light?
As noted above, the problem with bioluminescence is not in producing luciferase in vivo - I have a mammalian plasmid in my lab that can do that. The issue is producing and trafficking luciferin (luciferase's substrate).
Feb
18
comment How does Hemocytoblast constantly get formed into so many blood cells and yet remains there in the bone marrow throughout the lifetime?
@a.aniq yes, it's mitosis. What else would it be? Also, since these cells do remain active for an entire lifetime, your assumptions need to be re-evaluated.
Feb
18
comment What happens to the umblical cord inside the mother?
The umbilical cord is connected to the placenta, which is expelled shortly after birth.