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| bio | website | pigimal.com |
|---|---|---|
| location | Massachusetts | |
| age | 34 | |
| visits | member for | 11 months |
| seen | 2 hours ago | |
| stats | profile views | 9 |
Long-time OSS user/admin, beginning serious coder - I heart Python. Master of Science in Molecular Medicine, many years' experience in biotech working with antibodies and related assays. Science and computer geek. Dad.
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Apr 8 |
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Western blotting of lung samples I wrote the answer to the above question, please let me know if you're still having issues. Just leave a comment with @MattDMo in it. |
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Apr 8 |
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How do you store membrane proteins? You could always just add sodium azide and keep them at +4, so they don't have to go through a freeze/thaw at all. |
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Apr 8 |
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Chiral (a)symmetry of curly hair (and fur) @WYSIWYG a link might help the OP... |
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Apr 2 |
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EGFR, sialylation, and cancer progression @GPI - that's why I put "specific over-sialylation of EGFR could turn out to be very useful, if we can learn how to modulate and target it" in there. It's a pretty big if. |
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Mar 29 |
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205 nm UV-Vis readings What evidence do you have for the reasonableness of reading at 205? |
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Mar 29 |
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DNA gel extraction: chemical contaminants It could very well be that your digestion isn't proceeding very efficiently and you're just getting low yields. Do you have high A260 and A230 readings both? |
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Mar 28 |
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What is immunopanning (vs. immunoprecipitation and FACS)? @dd3 - no problem at all, I'm here to help. Thanks for checking it answered! |
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Mar 28 |
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What is immunopanning (vs. immunoprecipitation and FACS)? @dd3 - right. Also, you don't have the beads physically interacting with each other in the tube, potentially crushing or tearing cells. A cell could easily be tightly bound to two beads, one on either side, and possibly ripped apart when they separate. It's also much easier to remove cells bound to a plate than it is to separate the cells from the beads after the wash steps. |
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Mar 27 |
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What is background binding? can you post a link to the article? |
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Mar 23 |
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Why is important to get saturated fatty acids from diet? Where does it say that we must get saturated fatty acids from our diet? If they are not essential fatty acids, then by definition we don't need them in our diet - we can make them from other precursors. |
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Mar 19 |
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ChIP-seq and the output of SPP if you type in ??spp at the R prompt you'll get the docs. They're not expansive, but they explain the different parts of the library. |
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Mar 19 |
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ChIP-seq and the output of SPP does the inline spp documentation( > ??spp) explain it any better? |
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Mar 11 |
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What is a “tool strain”? Is there anyone else you can talk to? :) In my mind, recombinase strains could be a subset of tool strains, as a recombinase like Cre mentioned below is certainly a tool for genetic engineering, there are other "tools" that can also be used. |
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Mar 10 |
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What exothermic reaction distinguishes warm blooded animals? @Pete - Exactly. |
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Mar 9 |
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What exothermic reaction distinguishes warm blooded animals? The heat comes from the release of the proton gradient - it's just being changed from electrical energy to heat energy. I don't know if there's a specific chemical reaction going on... |
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Feb 27 |
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Specific enzyme for C. elegans Do you just need a unique protein/DNA sequence, and how unique and how long does it need to be? What exactly is the aim of your project? A common problem is that protein/enzyme names may not be identical between species, but their functional domains and/or sequences could very well be, since many processes of life are conserved from one species to the next. |
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Feb 16 |
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Does GTP-γS (GTP gamma S) bind all GTP-binding proteins? I can still see the figures, though. Which one did you have the question about? |
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Feb 16 |
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Does ethanol destroy RNase? The heat treatment is called depyrogenation and not only completely removes all biological activity, it also destroys pyrogens (fever inducers) such as LPS or endotoxin. |
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Feb 13 |
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Does GTP-γS (GTP gamma S) bind all GTP-binding proteins? If you can add a link to the article (hopefully it's open-access) that would be helpful in interpreting the results. |
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Feb 13 |
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Resuspending Cells from a filter plate Have you tried trypsinizing them? Depending on whether they're suspension or adherent, and the time they've been on the membrane, you might be able to detach them just like you would from a culture dish/flask. Also, are you using a vacuum to adhere them to the membrane, or just allowing them to settle? Too much pressure may be getting them caught in the pores, although theoretically the pores should be much too small for them to fit through... |
