Andrei Kucharavy

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visits member for 2 years, 2 months
seen Jul 15 at 21:15

Mar
3
accepted What are some of the known fungi with heat resistant spores
Mar
3
asked What are some of the known fungi with heat resistant spores
Jun
28
awarded  Editor
Jun
28
revised Can extinct animals be cloned?
added 134 characters in body
Jun
1
answered Can extinct animals be cloned?
Mar
21
comment E. coli values for [enzymes], [metabolites] and kinetic rates
This work was based on this paper and I had an impression that the authors run into pretty much the same limitations: only qualitative analysis is provided with the computational model, whereas they could have gone quantitative with this model and had quantitative experimental results.
Mar
21
comment E. coli values for [enzymes], [metabolites] and kinetic rates
This is my personal experience. When you use the COBRA toolbox, you use an external module for linear optimization to get your "objective" function. When I computed the base model from iAF1260 E.Coli, it worked well, just as a system of two bacteria living together. But as soon as you started to perform single or double gene deletion analysis, your objective function maximum started to behave erratically. You could make it bit better with it by using a different external linear optimization module, but still no analysis was possible.
Mar
19
answered E. coli values for [enzymes], [metabolites] and kinetic rates
Mar
19
answered Looking for a cancer drug target database to guide sequencing of patient tumor DNA
Mar
19
awarded  Supporter
Nov
27
awarded  Teacher
Nov
27
answered Analysing the results of real-time PCR
Nov
27
comment T7 phage promoter action in mammals
Thanks for the answer!
Nov
27
awarded  Scholar
Nov
27
accepted T7 phage promoter action in mammals
Jun
4
awarded  Student
Jun
3
asked T7 phage promoter action in mammals