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| bio | website | |
|---|---|---|
| location | Memphis, TN | |
| age | 31 | |
| visits | member for | 1 year, 5 months |
| seen | Apr 25 at 20:40 | |
| stats | profile views | 16 |
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Feb 2 |
comment |
Does an annealing temp higher than primer's Tm contribute to primer dimer? Ironically this is one of the few times were I didn't design the primers and used what was mentioned in numerous papers. |
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Feb 2 |
comment |
Does an annealing temp higher than primer's Tm contribute to primer dimer? I like your example and calculation. I guess I didn't think Tm was so polymerase specific before. Coincidentally it ends up at the same annealing temperature that I tried though. Unfortunately the taq I am using gives very little information (and not an online calculator) in regards to choosing temperatures. I am close to ordering a new mastermix anyway, so I will likely try a different brand then. But as for my current problem there are enough variables that I plan to try the same annealing temperature again and if that fails completely then I may look at what @jp89 suggested also. |
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Feb 1 |
comment |
Does an annealing temp higher than primer's Tm contribute to primer dimer? Using a Maxime PreMix which includes Intron's i-Taq |
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Jan 31 |
asked | Does an annealing temp higher than primer's Tm contribute to primer dimer? |
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Jan 11 |
awarded | Supporter |
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Jan 11 |
comment |
Alternatives to ethidium bromide for staining small nucleic acids? Interesting. I haven't done a comparison so I can't speak one way or the other. But I do remember a similar article sometime back saying a similar thing (EtBr is not as bad as you think, and SYBR Green really isn't a "safer alternative" as advertised). |
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Jan 4 |
answered | Alternatives to ethidium bromide for staining small nucleic acids? |
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Dec 29 |
awarded | Teacher |
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Dec 29 |
answered | What conditions should I use for Gel Red staining? |
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Dec 28 |
awarded | Student |
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Dec 28 |
asked | Are these Gram stain substitutions acceptable? |
