380 reputation
29
bio website
location Memphis, TN
age 32
visits member for 2 years, 7 months
seen Apr 23 at 20:30

Feb
16
comment X chromosome “weight”?
Girls take note, this is not a valid excuse. Seriously though, this difference is exploited in some (albeit minimally effective) techniques to sort sperm for gender selection.
Feb
2
comment Does an annealing temp higher than primer's Tm contribute to primer dimer?
Ironically this is one of the few times were I didn't design the primers and used what was mentioned in numerous papers.
Feb
2
comment Does an annealing temp higher than primer's Tm contribute to primer dimer?
I like your example and calculation. I guess I didn't think Tm was so polymerase specific before. Coincidentally it ends up at the same annealing temperature that I tried though. Unfortunately the taq I am using gives very little information (and not an online calculator) in regards to choosing temperatures. I am close to ordering a new mastermix anyway, so I will likely try a different brand then. But as for my current problem there are enough variables that I plan to try the same annealing temperature again and if that fails completely then I may look at what @jp89 suggested also.
Feb
1
comment Does an annealing temp higher than primer's Tm contribute to primer dimer?
Using a Maxime PreMix which includes Intron's i-Taq
Jan
31
asked Does an annealing temp higher than primer's Tm contribute to primer dimer?
Jan
11
awarded  Supporter
Jan
11
comment Alternatives to ethidium bromide for staining small nucleic acids?
Interesting. I haven't done a comparison so I can't speak one way or the other. But I do remember a similar article sometime back saying a similar thing (EtBr is not as bad as you think, and SYBR Green really isn't a "safer alternative" as advertised).
Jan
4
answered Alternatives to ethidium bromide for staining small nucleic acids?
Dec
29
awarded  Teacher
Dec
29
answered What conditions should I use for Gel Red staining?
Dec
28
awarded  Student
Dec
28
asked Are these Gram stain substitutions acceptable?