375 reputation
28
bio website
location Memphis, TN
age 32
visits member for 2 years, 4 months
seen Apr 25 '13 at 20:40

Feb
1
comment Alternatives to TBE buffer for denaturing polyacrylamide gel electrophoresis of DNA and RNA?
I've used Lithium Borate with an agarose gel and high voltages (>200). Didn't turn out as well as advertised the few times I tried it though - worse than TAE. If I had to try it again I would definitely lower the voltage.
Feb
17
comment Is there a cheap and easy way to check for potentially unsafe UV exposure?
That does seem obvious after looking at products available in a google search. Maybe one the reusable ~6 level color changing sensors would be adequate or even one of the ~$30 handheld digital models. Spending several hundred dollars is out of the question though. I was just hoping someone would say, oh yea all labs in the world use this cheap x sensor to warn us of excessive exposure (analogous to radiation badges).
Feb
17
comment Is there a cheap and easy way to check for potentially unsafe UV exposure?
Agreed, I'm still below the threshold for creating tags. Safety or visualization would have been remotely closer.
Feb
16
comment X chromosome “weight”?
Girls take note, this is not a valid excuse. Seriously though, this difference is exploited in some (albeit minimally effective) techniques to sort sperm for gender selection.
Feb
2
comment Does an annealing temp higher than primer's Tm contribute to primer dimer?
Ironically this is one of the few times were I didn't design the primers and used what was mentioned in numerous papers.
Feb
2
comment Does an annealing temp higher than primer's Tm contribute to primer dimer?
I like your example and calculation. I guess I didn't think Tm was so polymerase specific before. Coincidentally it ends up at the same annealing temperature that I tried though. Unfortunately the taq I am using gives very little information (and not an online calculator) in regards to choosing temperatures. I am close to ordering a new mastermix anyway, so I will likely try a different brand then. But as for my current problem there are enough variables that I plan to try the same annealing temperature again and if that fails completely then I may look at what @jp89 suggested also.
Feb
1
comment Does an annealing temp higher than primer's Tm contribute to primer dimer?
Using a Maxime PreMix which includes Intron's i-Taq
Jan
11
comment Alternatives to ethidium bromide for staining small nucleic acids?
Interesting. I haven't done a comparison so I can't speak one way or the other. But I do remember a similar article sometime back saying a similar thing (EtBr is not as bad as you think, and SYBR Green really isn't a "safer alternative" as advertised).