Timeline for What's the best way to purify my His tagged protein? Supernatant super viscous after first sonication?
Current License: CC BY-SA 4.0
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Apr 12, 2022 at 23:36 | comment | added | canadianer | It sounds like your protein is expressed in inclusion bodies. If that is the case, you shouldn’t need to worry about chromosomal DNA since it is rather trivial to wash away before solubilization. Be sure to centrifuge and filter your sample before applying it to the column to remove any insoluble debris. | |
Apr 12, 2022 at 21:00 | history | tweeted | twitter.com/StackBiology/status/1513985310853586953 | ||
Apr 11, 2022 at 12:34 | history | became hot network question | |||
Apr 11, 2022 at 12:20 | history | edited | MattDMo | CC BY-SA 4.0 |
added 4 characters in body; edited title
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Apr 11, 2022 at 12:13 | answer | added | MattDMo | timeline score: 5 | |
Apr 11, 2022 at 6:16 | comment | added | Chris♦ | Do you have DNAse in your buffers? This is most likely the chromosomal DNA which is causing problems here. Depending on how harsh you sonicate it should break down, but if you sonicate short and with low energy this must not happen. | |
Apr 11, 2022 at 4:41 | history | edited | Maximilian Press | CC BY-SA 4.0 |
cleaned up formatting etc.
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S Apr 11, 2022 at 4:33 | review | First questions | |||
Apr 11, 2022 at 12:26 | |||||
S Apr 11, 2022 at 4:33 | history | asked | Yoshokie | CC BY-SA 4.0 |