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Apr 12, 2022 at 23:36 comment added canadianer It sounds like your protein is expressed in inclusion bodies. If that is the case, you shouldn’t need to worry about chromosomal DNA since it is rather trivial to wash away before solubilization. Be sure to centrifuge and filter your sample before applying it to the column to remove any insoluble debris.
Apr 12, 2022 at 21:00 history tweeted twitter.com/StackBiology/status/1513985310853586953
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Apr 11, 2022 at 12:20 history edited MattDMo CC BY-SA 4.0
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Apr 11, 2022 at 12:13 answer added MattDMo timeline score: 5
Apr 11, 2022 at 6:16 comment added Chris Do you have DNAse in your buffers? This is most likely the chromosomal DNA which is causing problems here. Depending on how harsh you sonicate it should break down, but if you sonicate short and with low energy this must not happen.
Apr 11, 2022 at 4:41 history edited Maximilian Press CC BY-SA 4.0
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S Apr 11, 2022 at 4:33 history asked Yoshokie CC BY-SA 4.0