Timeline for Splice in with CRISPR/Cas
Current License: CC BY-SA 3.0
3 events
| when toggle format | what | by | license | comment | |
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| Mar 31, 2015 at 14:04 | comment | added | user137 | The second link is about homologous recombination. CRISPR and zinc-finger nucleases are just about cutting the DNA, homologous recombination is how the new gene is actually inserted. And I don't think you're going to easily push the CRISPR success rate above 50% of all cells, the recombination rate will probably be lower. A good selection strategy can find those recombined cells and give you a population with 100% recombination. | |
| Mar 31, 2015 at 8:57 | comment | added | Robertos | Thank you, but I don't ask how to separate the cells. I ask how to force Crispr to insert in higher success rate. How to make him less specific or what regions to target. If it's possible. And your second link about Zinc-finger nucleases and not Crispr. | |
| Mar 30, 2015 at 18:46 | history | answered | user137 | CC BY-SA 3.0 |