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We have an algal enhancer element and a transcription factor that probably binds it. Basal expression of the enhancer element or full promoter driving luciferase in tobacco protoplast was extremely low. I heard this could be overcome by fusing it to a minimal 35S promoter fragment but I can't seem to find a clear sequence for this minimal promoter.The best I could find was on the cambia website where they say it is -90 http://www.cambia.org/daisy/promoters/242/g1/250/264.html

1) Does anybody have experience with increasing the expression of enhancer elements like this? 2) And what sequence did you use for this?

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  • $\begingroup$ Apparently some other plant vectors define it as -46 to +9 relative to the start codon. Even more confused now: books.google.co.uk/… $\endgroup$
    – mimat
    Commented May 29, 2015 at 14:25

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In case anybody ever has the same question: I found the answer in this paper by Ow et al. 1987 (fig1) http://www.pnas.org/content/84/14/4870.full.pdf

Just to clarify, I did not want to boost expression of the enhancer element in my algae, it needed boosting in tobacco protoplasts/leaves.

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