I have done IFA (immunofluorescence assay) previously but i targeted only one protein or i tagged my endogenous protein with GFP/RFP then i performed IFA. I want to do the IFA in parental cell line (HEK cells). My aim is to show the two proteins are co-localized or not?
- Protein-1 (secondary antibody Mouse)
- Protein-2 (secondary antibody Rabbit)
How can i co-localize two different proteins in the same sample? Which primary antibody do i have to add first? can any one please suggest me something?