I am studying the procedures of forming hybridoma cells for generating a large number of monoclonal antibodies.
Before the procedure of fusion (with multiple myeloma cells) happens, I would like to ask how to ensure all the plasma cells in the cell extract only contains those which produce monoclonal antibodies only but not polyclonal antibodies?
I have searched in Wiki, and it says
identifying antigen-specific plasma/plasmablast cells (ASPC) that produce antibodies specific to an antigen of interest
I would like to ask what methods are available for us to do this identification?