I have to check if some genes are amplified with bioinformatics tools, but, in order to do it, I need to know what this means from a biological point of view.
It seems not so difficult to understand, in fact the definition from NIH is simply the following:
Gene amplification is an increase in the number of copies of a gene sequence. Cancer cells sometimes produce multiple copies of genes in response to signals from other cells or their environment. The term also can refer to polymerase chain reaction (PCR), a laboratory technique that is used by scientists to amplify gene sequences in a test tube.
So my question is: if I have the complete DNA sequence of the organism's genome that I am studying (like this), that belongs to Archaea domain, what I should do to check for gene amplification is to see if the sequence of the gene (that I suspect that it is amplified) is repeated more times ?
I thank you in advance.