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I want to make a DNA vaccine for fish by using plasmid pcDNA3.1(+) with a bacterial antigen. Unfortunately, there is a Kozak sequence in the middle of my target gene.

I am concerned that this Kozak sequence will interfere with translation inside fish cells.

Can I still use this gene of interest to make a DNA vaccine? Is there any way to deal with this unwanted Kozak sequence?

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    $\begingroup$ You could always try codon editing to interrupt the Kozak sequence, yet still have the same protein sequence. $\endgroup$
    – MattDMo
    Jun 23 at 17:44
  • $\begingroup$ Thank you so much @MattDMo, such a good idea to try :)) $\endgroup$ Jun 24 at 12:39
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    – tyersome
    Jun 27 at 18:03

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