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I'm trying to find a way to stop the transcription bubble from occurring over a individual gene in humans. I need the act of transcription to be inhibited, siRNA knock down of the transcript does not fit the bill. I'm unsure how to handle this problem and looking for suggestions.

My current approach is to look for a gene that has it's transcription entirely dependent on a single transcription factor. This would allow me to knock down the transcription factor to prevent the gene being transcribed in the first place. The problem is finding these genes and proving that they are not also under the influence of another unknown TF.

Is this a good approach? Is there a better way? And how would I find genes controlled in that manner in the first place?

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    $\begingroup$ Hi James, and welcome to Biology.SE! Your question reads like an XY problem. It would be helpful if you could provide details concerning why you want to target a single (but not a specific) gene, and why siRNA would not work. Have you considered mutating the promoter or TF binding sites? If you do find a gene under control of a single TF, consider that knocking down the TF will also affect the other genes it regulates $\endgroup$
    – acvill
    Jun 28 at 14:07

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