Does adding the overhangs to gBlocks for NEB Hifi using PCR pose a big problem? The IDT website suggests that one should design the gBlocks with the overhangs. However, if I did not do this, will it be a problem?
Edit: I intent to use Q5 High-Fidelity Polymerase and the gBlocks range from 500-2200 bp in size. It's not necessary for the exact sequence to be present.