I am trying to create a long-term stock for Bacillus subtilis (Bacterial glycerol stocks). I have looked online for guides and step-by-step tutorials and I found that they don't say where the sample originally comes from. In my case, I am using Bacillus subtilis subsp. subtilis (ATCC® 23857™) pure from the supplier bottle.
What is the proper procedure to create a long-term stock for Bacillus subtilis using this product?