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I have a sequence ATG GGG CCC TTT AAA TAA and want to use it as antisense RNA in my vector. How should I clone it? I am confused with the direction of my clone. I looking for the direction and sequence of the clones.

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  • $\begingroup$ Perhaps place a promoter or ori upstream in the antisense direction? $\endgroup$ – Atticus29 Sep 8 '13 at 15:40
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Tour sequence's complementary is TAC CCC GGG AAA TTT ATT. Together they form a DNA strand. Now replicate this and then, after transcription of both the DNA you get the RNA sequence you wanted. Use it as sense or antisense.

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  • $\begingroup$ Is there something missing from "transcription of both the DNA"? $\endgroup$ – kmm Sep 10 '13 at 12:17

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