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Let's say I have a picture of an insect wing and a ruler on the same image. Is there any computer program that I can use to calibrate with the ruler and then get a measurement on the wing length? Would the software be able to export these data to a spreadsheet?

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    $\begingroup$ I'm voting to close this question as off-topic because this question is about software not Biology. $\endgroup$ – dustin Apr 20 '15 at 20:43
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ImageJ is all you need. Particularly see the documentation sections on setting the image scale and measuring.

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  • $\begingroup$ This is great! Thanks! It even continues to add values from new images into the table. Awesome! $\endgroup$ – Atticus29 Sep 11 '13 at 17:57
  • $\begingroup$ see also Fiji for a distribution of ImageJ with more plug-ins $\endgroup$ – Michael Kuhn Sep 12 '13 at 11:28
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WebPlotDigitizer is an online application that can also be used to make distance and angle measurements in an image.

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  • $\begingroup$ Looks interesting. I will try it at the next chance. $\endgroup$ – Chris Jun 24 '14 at 10:32

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