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When visualising gene expression, you can use UAS-GAL4 to drive expression of GFP in all cells that express the protein and then stain the cell with anti-GFP. But why do this when you could just directly stain against your protein of interest?

I saw a paper that adopted both these methods and highlighted which cells where marked by both approaches- what does it mean if a cell is only marked by one?

Thanks for your help!

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    $\begingroup$ Please explain what you mean by "directly stain against your protein of interest". If you mean react with an antibody to the protein the answer should be fairly obvious that you generally don't have one. If the authors of the "paper you saw" did have such an antibody you need to give a reference to it so that anyone who is so minded to can help you (and the way you give thanks for help on this site is to accept or upvote and answer, not to include it in your question). $\endgroup$
    – David
    Sep 20 at 17:20