could someone help me to understand the following sentence better? It's from the book "A Practical Guide to Assay Development and High-Throughput Screening in Drug Discovery". And it's about fluorophors used in FRET-assays, for screening of protease inhibitors.
"In contrast to many other FRET dye pairs, both EDANS & DABCYL and Mca/Dnp generate a dynamic range between cleaved an uncleaved substrate, sufficent for the application of such protease assays in HTS and high-throughput compound profiling under initial conditions. "
I'm confused about the last part of the quote "under initial conditions". What does that mean? the question summarized "What is the dynamic range under initial conditions? "