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I am wondering whether we can replace 70% ethanol to fix and perm the cells with 4% PFA to observe the cell cycle. Do I understand correctly that 4% PFA can permeate the cells, thus the PI will intercalate DNA?

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  • $\begingroup$ PFA can also cross-link DNA, interfering with intercalation. $\endgroup$
    – MattDMo
    Commented Nov 21, 2021 at 20:05
  • $\begingroup$ Thanks, I will search more details. $\endgroup$
    – tassaneel
    Commented Nov 13, 2022 at 11:51

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