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I'm looking at expressing a protein in saccharomyces cerevisiae using the Adh2 promoter. My understanding is that the gene will be repressed by the presence of glucose, but when glucose runs out it should be activated. If my strain has deletions in ethanol biosynthesis, will Adh2 promoter still work?

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  • $\begingroup$ Why wouldn't it? As long as you haven't deleted the transcription factor(s) that bind to the Adh2 promoter, it should work just fine. $\endgroup$
    – MattDMo
    Mar 25, 2022 at 23:43
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    $\begingroup$ I'm coming across publications talking about ethanol encouraging dimerization of the transcription factor into it's active state, and I just wanted confirmation if ethanol is necessary. $\endgroup$ Mar 27, 2022 at 1:55
  • $\begingroup$ Ah. In that case, feel free to ignore what I said and do more research. I don't know much about Adh2 specifically (although I'm fairly well informed about transcription factors in general), so you'll have to see if the promoter is absolutely dependent on EtOH, or if that's just one way it's activated. $\endgroup$
    – MattDMo
    Mar 28, 2022 at 12:51

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