I would like to use the plasmid pXen5 (by Xenogen) for a transposon screen.

It contains two inverted repeat sequences, with Luciferase, Kanamycin, and the transposase itself in between. (It's tn1409).

Is it a problem to use such a transposon, which contains the transposase within the IR regions?

I am concerned that because the transposase will always be expressed (since its coding sequence is carried around inside the transposon), the transposon will keep on "jumping".

How real is this problem from a practical point of view?

Thanks, J



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