I have some samples of whole blood that are a little bit expensive and I want to significantly reduce the concentration of PPi in the samples by causing a reaction. I don't have any experience in practical chemistry, but I understand that in theory, there are enzymes that can hydrolyze pyrophosphate into orthophosphate (e.g. inorganic pyrophosphatase).
I see that I can purchase inorganic pyrophosphatase suspended in 3.2 M ammonium sulfate solution, (pH approximately 6), from yeast, on Sigma-Aldrich and in theory converts 1 micromol pyrophosphate to orthophosphate per minute at room temperature (in the presence of Mg-ions). For my purpose, the reduction in pH is not a problem. I am more concerned about whether PPi concentration would actually go down, or if I could expect cell lysis or other effects to occur, causing PPi to rise.
Can I use this reagent to reduce the measurable PPi concentrations in the blood samples? And if so, how would I do it?
EDIT: I am doing an experiment with bacterial growth in plasma, and comparing with serum deficient in pyrophosphate. I want to have a rough idea of the appropriate way to hydrolyse the PPi.