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I am working on designing a lab for an undergrad genetics course which involves dissecting and visualising polytene chromosomes from Drosophila salivary glands. I need a non-fluorescent non-antibased staining for the DNA to visualize the chromosomes, as we won't have access to antibodies or an episcope (only compound microscopes). Does anyone know of a DNA dye that could work?

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Giemsa stain should work. It is traditionally used in karyotyping staining. It results in G-banding.

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