I'm researching a polymorphism using restriction fragment length polymorphism (RFLP) and gel electrophoresis.

After RFLP, I should see fragments at 141bp and 111bp, but I can not see in 2% agarose, what is your suggestion, dear friends?

Here is my gel recipe:

  • TBE: 0.5% 75ml
  • agarose: 1.5gr
  • Voltage: 65
  • Time: 1 hour

Unfortunately, I do not have the ability to change the methods.

  • 4
    $\begingroup$ Why can't you change the conditions? Anyways, fragments of 141 and 111 bp should separate in 1,5% agarose. Can you show an image of your gel?` $\endgroup$
    – Chris
    Jun 10 at 20:48
  • 2
    $\begingroup$ How are you visualizing the bands? Ethidium bromide or SYBR green or something? What does "1hr" mean, did you run it for 1 hour? Did you load a ladder to confirm that the band didn't run off. $\endgroup$ Jun 10 at 23:24


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