I'm researching a polymorphism using restriction fragment length polymorphism (RFLP) and gel electrophoresis.
After RFLP, I should see fragments at 141bp and 111bp, but I can not see in 2% agarose, what is your suggestion, dear friends?
Here is my gel recipe:
- TBE: 0.5% 75ml
- agarose: 1.5gr
- Voltage: 65
- Time: 1 hour
Unfortunately, I do not have the ability to change the methods.