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Here's what I understand and please correct me if I am wrong:

Plasmids modified for gene therapy or genetic engineering should contain factors for certain functions in prokaryotic cells. For example, it should contain an origin of replication for amplification in prokaryotic cell as bacteria.

In the same time, if it's being made to alter a mammalian cell, which is an eukaryotic cell, it should contain features that helps in expression of a certain gene as Kozak sequence

So is it right to say that we can divide features in the architecture of plasmids into features that serve prokaryotic functions and others that serve eukaryotic functions?

are these functions restricted to replication in prokaryotics and expression of genes in eukaryotics?

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    $\begingroup$ Plasmids are prokaryotic. If used as vectors for cloning they are still prokaryotic as regards their replication transcription and translation. The inserts in the vectors have the characteristics of the organism from which they originated. If the purpose is to introduce the insert into a eukaryote and hope to have it expressed then it will obviously require the transcriptional and translational signals to allow this occur, and these differ from prokaryotes to eukaryotes. You seem to understand this, so I don't quite see what your question is. $\endgroup$
    – David
    Jun 23 at 18:11
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    $\begingroup$ Not to be nitpicking, but while I agree that for cloning purposes plasmids are to be considered prokaryotic, eukaryotic plasmids do exist. The yeast 2-micron plasmid is one example (PMC213983). $\endgroup$
    – gaspanic
    Jun 23 at 18:47
  • $\begingroup$ @david i want to know if my reading is correct and also i want more specificty: are these functions restricted to replication in prokaryotics and expression of genes in eukaryotics? $\endgroup$
    – Themobisback
    Jun 23 at 23:51
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    $\begingroup$ Unfortunately I don’t think you have a specific biological question in the terms of this list. $\endgroup$
    – David
    Jun 24 at 7:03

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