PCR has three steps: Denaturation, Annealing, and Primer extension. Let's say you do not have a thermal cycler in the lab, how would you mimic similar conditions and perform PCR?

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    $\begingroup$ three water baths and patience $\endgroup$
    – acvill
    Commented Jul 6, 2022 at 13:21
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    $\begingroup$ Worth noting that three reasonably accurate water baths might cost more (and definitely take up more space) than a small, cheap thermal cycler $\endgroup$
    – MikeyC
    Commented Jul 6, 2022 at 16:14

1 Answer 1


Use of 3 water baths was the original method. Perhaps isothermal amplification an option? Many reagents change colour upon amplification to visually identify successful pcr, which could make screening faster.


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