What is the order in which this DNA samples would would reassociate ?

e.coli DNA

plasmid DNA

viral DNA

satellite DNA

DNA-RNA hybrid

This is a homework question and I have not been given any information about the condition in which they are reassociated.

  • $\begingroup$ RNA-DNA hybrids have a higher thermodynamic stability compared to DNA-DNA hybrids (deltaG(hybridization)) is low. However that doesn't really mean they will form faster. Just that the equilibrium is towards association. $\endgroup$
    Oct 24 '13 at 10:56

The rate is determined by the size and complexity of the sequence. Short, non-repetitive sequences should re-associate (at a given r.t.p.) faster than longer, repetitive DNA.

I'm guessing that that is what this question is getting at.


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