When a virus replicates, it has to create several copies of its genome to the "daughter viruses"? Where in the cell does this replication of the viral genome take place? And how?
In my book, they use HIV-viruses as an example, which I've understood is a retrovirus. The first steps they describe are attachment, entry and uncoating, which I understand. Then, the next step is integration, which they say start with that the RNA translates to DNA and the new DNA strand works as a template to create a dsDNA. dsDNA is then integrated in the host cells genome, and then after that the transcription and translation of virus protein can start. What I don't understand is when the replication of RNA strands take place? After the synthesis of protein, there is the assembly, where the protein and RNA build up new viruses. Where does the RNA come from? Does the replication take place before the integration? Or when the DNA is integrated in the host cell genome, at the same time as the protein synthesis?
For other types of virus, they say that the genome replicates by a "rolling circle mechanism"? Where does that take place? In the cytoplasm? In those cases, isn't the viral genome integrated in the host cells genome at all? They just use the host cells polymerase, ribosomes etc.? Is retro viruses the only viruses that integrates in the genome?
This became kind of long, but to summarize my questions:
- Retroviruses: Where and how does the replication of the RNA take place? Before or during integration of the host cell genome?
- Where does the replication of genome of other viruses (that use the rolling circle mechanism) take place? In the cytoplasm?