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For a project, I am trying to figure out if it is possible to determine the acidification of milk without a probe. I am fairly experienced with machine vision and sensors, but not even a little in biology. I read somewhere that "Milk spoils when bacteria convert the lactose into glucose and galactose, producing lactic acid." I was wondering if there is a way to detect these changes using a special camera or light (techniques) like NIR/Raman spectroscopy or UV.

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  • $\begingroup$ The easiest way would be to use a colored indicator in the milk that changes color with acidity. $\endgroup$
    – Chris
    2 days ago
  • $\begingroup$ Thank you for your suggestion, but I am looking for a no-probe/contact/destruction way to achieve this goal. I will edit my question to clarify this better. $\endgroup$
    – Malinko
    2 days ago
  • $\begingroup$ Thanks for the edit, but are we supposed to correct spelling in a quote? $\endgroup$
    – Malinko
    2 days ago
  • $\begingroup$ Supposed is probably said too much. I would have suspected a typo and corrected it as well. $\endgroup$
    – Chris
    2 days ago
  • $\begingroup$ If you're going to be using (I assume) a flow cell to do spectroscopy, why not just have a pH meter in the flow? $\endgroup$
    – MattDMo
    yesterday

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Speculation here.

The viscosity may slightly change. Perhaps detectable by vibrating the sample and checking the pattern of standing waves on the surface.

Note that acidification due to lactobacilli may well happen after spoilage due to other bacteria.

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