What are the possible methods to prevent the digestion of antibodies (mainly Polyclonal) by proteases during affinity chromatography?
I read some papers about doing modifications to the anitbodies:
using unnatural amino Acids using mPEG But, are there better options, more commercially applicable?
I am trying to find a way to purify hyaluronidase from hepatopancreas of red king crab (using polyclonal antibodies). In this case, inhibitors like EDTA, Benzamidine, PMSF don't prevent proteolysis. These are the Hepatopancreas proteases: (Collagenolytic Serine Proteinase PC (PC—Paralithodes Camtschaticus), Collagenolytic Serine Proteinase PC 2, Trypsin-Like Proteinase A, Chymotrypsin-Like Proteinase C, Aminopeptidase PC, Carboxypeptidase PC, Trypsin PC, Elastase, Cathepsin L, Metalloproteinase)