On every gel of this type that I've ran so far, I'm getting this large band interfering with my gel analysis no matter how much troubleshooting I've done. This is a Novex Wedgewell 6% Tris-Glycine Gel and in each well I've loaded 10ul of the Novex 2X Native Tris-Glycine Sample Buffer + 10ul of my sample. I ran the gel at 100V for 1hr at 4C. This image is from under a Fluorescein Blot filter.

I've troubleshooted the gel by pre-running it and also trying to load without any 2x loading buffer, but the band has still remained.

enter image description here



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