I need to extract total protein from 2mm skin biopsies for western blots.
I was thinking about
miRNeasy Micro Kit (50) Cat. #217084 while it's ok for total RNA extraction, I am not sure if it is still fine for proteins. There's a trizol (quiazol) step that provides you with organic fraction that suppose to contain protein fraction, but I am still not sure if it's a best way.
There's also a so-called
Urea Sample Buffer (USB), that contains urea, 20% SDS, glycerol, Tris and Pyronin Y - but I am not sure it's a good choice since the percentage of SDS is high and it will be a disaster if I will try to disrupt my tissue.
Or, maybe there's a better solution or kit for that purpose?