I am an undergraduate student of biology.

I saw a lecturer online, who said that sticky ends makes phosphodiester and hydrogen bonds cut. (also here).

But why blunt end just makes phospodiester bonds cut?

Thank you for answering.

  • 2
    $\begingroup$ Welcome to Biology StackExchange! Do you know how the structure of DNA looks like, and where phosphodiester and hydrogen bonds are located? Did your lecturer show you an image of sticky-ends and blunt-ends cuts? $\endgroup$
    – Domen
    Aug 3, 2023 at 21:21
  • $\begingroup$ Does this help? @ ncbi.nlm.nih.gov/books/NBK21129 $\endgroup$ Aug 4, 2023 at 18:09
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    $\begingroup$ @BrendanDarrer thank you for sharing with me, but unfortunately the book didn't contains the answer that i'm looking for. $\endgroup$
    – arsy
    Aug 4, 2023 at 18:35
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    $\begingroup$ @Domen hi, thank you for your warm welcome. Yes i know the structure of DNA looks like and where are the bonds located. My lecturer shows me how the sticky-ends and blunt-ends looks like using GAATTC strand (for sticky-ends) and CCCGGG strand (for blunt-ends). Can you please enlight me about this cause i really need to know. Thank you so much $\endgroup$
    – arsy
    Aug 4, 2023 at 18:36
  • $\begingroup$ Have you found the answer or are you still looking for one? In short - Hydrogen bonds occur between the 2 strands, whereas phosphodiester is within the each strand. so a sticky end has a few overhang nucleotides on one strand that are absent in the other strand, hence the hydrogen bond is missing.. $\endgroup$ Aug 16, 2023 at 21:15


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