I recently started to get involved with quantitative PCR. I have some data and I want to calculate the copy number of them. In the output Excel file, I have the following cells (I am running Quandstudio v.1.5.2).
Well| Well Position|Omit|Sample Name|Target Name|Task Reporter|Quencher|CT|Ct Mean|Ct SD|Quantity|Quantity|Mean|Quantity|SD|Y-Intercept|R(superscript 2)|Slope|Efficiency
I found the following formulas
copy number = 10^(ct - intercept)/(slope) copy number = 2^-ΔΔCt
I have two questions. a) Are these formulas accurate? b) Is there any other suggested formula that I can apply to my data?
If there is any helpful article that has calculation formulas, it is more than welcome!