1
$\begingroup$

I just received a primer (details are displayed in the picture). Since this is my first time doing this, I want to do it correctly. My goal is to make a 10µM primer. So, If I get it right and based on the paper I received, should I have to add 665μl Sterile distilled water (Inside the primer tube) and then aliquot 90μl Sterile distilled water with 10μl of primer tube mix? enter image description here

$\endgroup$

1 Answer 1

2
$\begingroup$

Yes, this is correct. Note that it is not important that the water has been sterilized, it needs to be nuclease free.

Also, for long term storage TE buffer might be a better choice. This will not result in inhibition of your PCR by the EDTA component of the buffer, since the amount of it in the final reaction is neglectable.

$\endgroup$
2
  • 1
    $\begingroup$ Thank you very much for the response as well as for the tip!!!! $\endgroup$
    – KGee
    Commented Oct 27, 2023 at 21:29
  • $\begingroup$ @KGee If this answer helped, make sure to accept the answer by clicking the checkmark $\endgroup$ Commented Oct 28, 2023 at 9:59

You must log in to answer this question.

Not the answer you're looking for? Browse other questions tagged .