I would like to test the proteins that bind to biotinylated-RNA by mass spectrometry , I was using Dynabeads™ MyOne™ Streptavidin C1 (invitrogen) coupled with biotinylated-RNA,than mix these beads up with cell lysate to achieve protein-RNA interacted solution, so does anybody know the better idea to elute that protein out of the mixed reagent?

  • 2
    $\begingroup$ What does the manual say about elution? $\endgroup$
    – Chris
    Nov 15, 2023 at 22:05
  • 1
    $\begingroup$ It doesn't have any info about elution $\endgroup$
    – Fino
    Nov 20, 2023 at 14:31
  • $\begingroup$ If your end goal is mass spec, you could do an on-bead digestion of the protein. Alternatively you could elute the protein with SDS buffer and do the digest in a gel. It should also be possible to break the protein-RNA interaction (eg with salt, cosolvents, and/or pH) $\endgroup$
    – canadianer
    Nov 20, 2023 at 22:15
  • $\begingroup$ any specific protocol or detail with it? $\endgroup$
    – Fino
    Nov 23, 2023 at 20:16


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