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I want to use the method of Single Cell Gel Electrophoresis (comet assay). I have many samples, but I don't have the appropriate microscope. So I have to preserve my slides until the time comes for staining and microscopic analysis. I've read many different protocols, and I can't decide which is the best. Also, after the staining and the analysis, I might need to reuse the same slide. Is that possible? and if yes, what should I do?

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  • $\begingroup$ Can you give more information about what protocols you have looked into? $\endgroup$ – kmm Nov 8 '13 at 13:26
  • $\begingroup$ Most of them use either 100% or gradually increasing percentage of ethanol or methanol. Some suggest to hydrate before the staining and some of them don't. Also some use mounting media at the end and others just a coverslip. I suppose that most of these work but it would be very helpful if I had a specific protocol that I know that will work. $\endgroup$ – Stella Nov 11 '13 at 9:22
  • $\begingroup$ This could be of use: onlinelibrary.wiley.com/doi/10.1002/cyto.a.20071/full $\endgroup$ – ktyagi Aug 5 '16 at 13:56

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