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I have been culturing SVG cell lines. This line was obtained as a live culture from another institute and was delivered by mail. After subculturing , I saw some particles, and I do not know what they are. The cells are growing well, no debris, no colour change, no foul smell. Can anybody tell me what this is? enter image description here

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    $\begingroup$ Welcome JEME. Could you edit to add some details like: 1) The magnification (or at least some idea of scale), 2) Which objects are you referring to, do you mean the crinkled object in the centre of field or something else? $\endgroup$ Commented Feb 22 at 17:44

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It is an imperfection in your plasticware.

Here's my reasoning:

  1. I've seen similar artefacts 3-4 times in 5 years of culturing mammalian cells and it doesn't move within the media on gentle agitation, indicating that it is fixed in place.
  2. it is extremely bright, and it is a phase contrast image. There's an object there but there is little refraction. I imagine light bouncing around inside a lump of distorted plastic.
  3. the artefact is huge compared to the cells (again with little contrast). I'd be surprised if it was any kind of organic aggregate as suggested in a previous comment.
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    $\begingroup$ Is it? Assuming it's the large object in the centre of the view, it looks like it is above the focal plane but still close to focus, which would indicate something other than plasticware - perhaps debris from the plasticware, or something like a protein precipitate from FBS $\endgroup$
    – bob1
    Commented Feb 27 at 20:01
  • $\begingroup$ Anything that you can add to support this or clarify what you mean? $\endgroup$ Commented Apr 18 at 16:55
  • $\begingroup$ I'm afraid not, but here's my reasoning: 1) I've seen similar artefacts 3-4 times in 5 years of culturing mammalian cells and it doesn't move within the media on gentle agitation, indicating that it is fixed in place. 2) it is extremely bright, and it is a phase contrast image. There's an object there but there is little refraction. I imagine light bouncing around inside a lump of distorted plastic. 3) the artefact is huge compared to the cells (again with little contrast) . I'd be surprised if it was any kind of organic aggregate as suggested in a previous comment. $\endgroup$
    – user80053
    Commented Apr 23 at 16:39
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    $\begingroup$ @user80053 There's nothing in the question to indicate 1) that it doesn't move, 2) it's a very poorly aligned phase contrast, looks like pseudo-phase by having the light source pointed a little sideways to the condensor. It is very rare to have obvious imperfections to the inside flat surfaces of the flask (and this is definitely inside based on focus), but not uncommon to have protein precipitates from FBS and/or plastic debris inside the flask from manufacture. 20+ years of cell culture tells me this... $\endgroup$
    – bob1
    Commented Apr 25 at 1:19

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