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I am trying to identify secondary metabolites gene clusters specifically PKS from endophytes through PCR then sanger sequencing. Are there set of specific primers available or do I have to design my own?

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  • $\begingroup$ What fungal species is it ? Are there primers in the literature designed by people who study the same secondary metabolites as you ? What aspect of the gene clusters do you want to study ? $\endgroup$
    – CaroZ
    Commented Feb 28 at 8:52

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