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I am looking for references to papers containing the time intervals spent in different phases of the cell cycle (ej., G0, G1, S, G2, M for eukaryotes) for different cells. In particular, I am interested in E. coli and CHO (Chinese Hamster Ovary cells), but any reference to studies of this kind for any typical cell will be useful.

I'll accept an answer containing a representative sample of references to the literature on this subject. Preferably recent papers (since 2010).

If you can provide the times spent in each phase but don't have references at hand, that will also be useful.

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    $\begingroup$ As far as I am aware the cycle stages that you list don't apply in E. coli, or they certainly didn't when I followed this. See nature.com/nrmicro/journal/v7/n11/fig_tab/nrmicro2202_F1.html for a recent diagram. C is 40 min and D is approximately 20 min. Cycles can of course overlap in E coli so that in a cell currently part way through a C phase a new C phase can be initiated concurrently. This is why E. coli can have a doubling time that is less that the length of a cell cycle. $\endgroup$ – Alan Boyd Nov 29 '13 at 14:37
  • $\begingroup$ @AlanBoyd You're right. These phases don't apply to E. coli. Editing... $\endgroup$ – becko Nov 29 '13 at 16:19
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I had done some search on this before but not for CHO (I checked for the cells that I was culturing). I can look up for more. Just pasting the data that I have right now.

Cell type   Total      G1      S      G2      M      Ref
----------------------------------------------------------
Neuro2a        9       2       5      1.5    0.5     [1]
Hela         16.2     7.7     7.2     0.8    0.5     [2]
A549          18       7      7.5     2.5     1      [3]
MCF7         21.3      9      9.3      2      1      [4]
CHO           15       6      6.8     1.2    0.65    [5]

The timescale is in hours.


References:

  1. De Laat et al. 1980
  2. Kumei et al. 1989
  3. Orfanoudakis et al. 1989
  4. Taylor et al. 1983
  5. Harada and Morris 1981
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  • $\begingroup$ +1 Thanks. This is useful, but I am looking for recent papers mostly. If you provide some more recent references (from 2010, preferably reviews), I'll accept this answer. Also, do you have any data on CHO or NS0? And what are the units of time in this table (hours, days)? $\endgroup$ – becko Nov 30 '13 at 15:21
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    $\begingroup$ its not a popular thing for researchers to reproduce old results... not sure you will find more recent ones. $\endgroup$ – shigeta Dec 2 '13 at 4:12
  • $\begingroup$ yeah actually while i was looking this up I couldnt find recent papers. Too obsolete for current times i assume :P I'll try to look up. $\endgroup$ – WYSIWYG Dec 2 '13 at 4:22
  • $\begingroup$ @shigeta so you're saying that these measurements have not been significantly improved/corrected in 20 years? $\endgroup$ – becko Dec 2 '13 at 18:01
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    $\begingroup$ Not definitively, but its quite possible. I'm sure we could measure melting points and osmolarity better now, but I can't recall seeing someone spending their time to do it. Science famously doesn't even try to reproduce some of their most important cancer research. I wouldn't fault WYSIWYG if they can't be found. $\endgroup$ – shigeta Dec 2 '13 at 18:06

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